Laboratory of Molecular Biology, Biochemistry and Biophysics

Laboratory of Molecular Biology, Biochemistry and Biophysics (LMBBB) is dedicated to research and teaching activities.

The main research facilities include equipment designed for basic and advanced biochemical and biophysical analyses of microbial, plant and animal cell compounds. Separation, quantitative and qualitative measurements of proteins, carbohydrates, lipids and nucleic acids can be performed at our premises employing spectrophotometers, spectrofluorimeter, chromatographic and gel-electrophoresis units. Molecular investigations of genotypic and phenotypic features of living cells can be achieved by PCR, IEF, Western blotting and protein array techniques. Additionally, animal and cell cultures facilities and expertise are available at the premises of our research laboratory.

Research interests/fields:

  • biomedical biochemistry;
  • bionanomedicine;
  • environmental molecular biology;
  • molecular diversity and taxonomy;
  • cell physiology.

 Domains of activity

  • Animal cell culture:
  • Genomics, proteomics and NGS data analysis;
  • Microbial cell culture;
  • Biochemical analyses.

Staff

General biochemistry, immunobiology and bionanotechnology

  • Assoc. Prof. Manuela BANCIU, PhD
  • Lecturer Emilia LICĂRETE, PhD
  • Lecturer Alina SESĂRMAN, PhD
  • Teaching assistant Laura PĂTRAȘ, PhD student

Biophysics, bioinformatics, molecular enviornmental microbiology

  • Prof. Horia BANCIU
  • Teaching assistant Paul-Adrian BULZU, PhD

Major infrastructure

 

Optical microscope model Axio Scope A1 with fluorescence module and color Axio Cam 105 camera (Carl Zeiss, Germany)

Specifications: Axio Scope A1 Microscope HAL 50, FL-LED, 3x H Microscope, 3x DIC with Fluorescence Module (365 nm and 540 nm fluorescence), Axio Cam 105 color camera with CMOS sensor, 5 Mpixels resolution

Uniqueness: Optical microscope with multiple functions (direct light, phase contrast, dark field, fluorescence)

Applicability: cell count, cell morphology, cellular structures (fungal, animal and plant cells), cell viability assessment.

Fields: environmental and medical microbiology, cell biology, animal cell cultures, hematology, animal / plant histology, histopathology.

Teaching provided: Biophysics (undergraduate – Biology, Biochemistry); Modern Biochemical and Biophysical Methods (Master – Molecular Biotechnology)

Persons in-charge:

  • Prof. Horia Banciu – horia.banciu[a]ubbcluj.ro
  • Lecturer Dr. Emilia Licărete – emilia_licarete[a]yahoo.com
  • Teaching Assist. Laura Pătraș – patras.laura88[a]yahoo.com

Access: Mo to Fri, 8-17; with e-mail programming addressed to one of the specialists listed above.

 

CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad, USA)

Specifications: C1000 Touch ™ thermocycler model with CFX96 ™ optical module (Bio-Rad, Hercules, CA, USA). Optical detection: Excitation 6 filtered LEDs; Detection of 6 filtered photodiodes; Range of excitation / emission wavelengths 450-730 nm; Detection sensitivity 1 copy of the target genomic DNA sequence; dedicated software for data analysis.

Applicability: amplification and quantification of gene sequences, melting curve analysis, gene expression analysis, allelic discrimination.

Fields: molecular biology, genetics, molecular diagnosis, genomics, transcriptomics.

Teaching provided: Biochemistry of nucleic acids with genomic elements (undergraduate – Biochemistry); Structure and evolution of the genome (Master – Molecular Biotechnology); Environmental Molecular Biology (PhD – Doctoral School of Integrative Biology).

Persons in-charge:

  • Prof. Horia Banciu – horia.banciu[a]ubbcluj.ro
  • Lecturer Dr. Alina Sesărman – alina.sesarman[a]ubbcluj.ro

 Access: Mo to Fri, 8-17; with e-mail programming addressed to one of the specialists listed above.

 

Server System Intel R1304WFTYS WOLF PASS (Intel, USA)

Specifications: Processor Xeon® Gold 6252 24C/48T 150W 2.10G 35.75M 10.40GT/sec ITT, Memory 12 X 64GB_LRDIMM_2666

Applicability: NGS data analysis, genome assembly, phylogenomics, comparative genomics,  metabolic reconstruction

Fields: advanced bioinformatics, molecular biology, molecular ecology, biodiversity, medical genomics and proteomics, interactomics.

Teaching provided: Biochemistry of nucleic acids with genomic elements & Introduction to Bioinformatics  (undergraduate – Biochemistry); Structure and evolution of the genome & Bioinformatics (Master – Molecular Biotechnology); Environmental Molecular Biology (PhD – Doctoral School of Integrative Biology).

Persons in-charge:

  • Prof. Horia Banciu – horia.banciu[a]ubbcluj.ro
  • Teaching Assist. Dr. Paul-Adrian Bulzu – bulzupaul[a]gmail.com

 Access: Mo to Fri, 8-17; with e-mail programming addressed to one of the specialists listed above. Remote access of the server is possible upon providing the appropriate username and password.